imaging filter Search Results


post column imaging energy filter  (Gatan Inc)
98
Gatan Inc post column imaging energy filter
Post Column Imaging Energy Filter, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/post column imaging energy filter/product/Gatan Inc
Average 98 stars, based on 1 article reviews
post column imaging energy filter - by Bioz Stars, 2026-06
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incucyte s3  (Sartorius AG)
99
Sartorius AG incucyte s3
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Incucyte S3, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/incucyte s3/product/Sartorius AG
Average 99 stars, based on 1 article reviews
incucyte s3 - by Bioz Stars, 2026-06
99/100 stars
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biocontinuum imaging filter  (Gatan Inc)
99
Gatan Inc biocontinuum imaging filter
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Biocontinuum Imaging Filter, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biocontinuum imaging filter/product/Gatan Inc
Average 99 stars, based on 1 article reviews
biocontinuum imaging filter - by Bioz Stars, 2026-06
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emission 520  (Revvity)
91
Revvity emission 520
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Emission 520, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/emission 520/product/Revvity
Average 91 stars, based on 1 article reviews
emission 520 - by Bioz Stars, 2026-06
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emission filter 500  (Revvity)
90
Revvity emission filter 500
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Emission Filter 500, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/emission filter 500/product/Revvity
Average 90 stars, based on 1 article reviews
emission filter 500 - by Bioz Stars, 2026-06
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530 emission filters  (Revvity)
90
Revvity 530 emission filters
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
530 Emission Filters, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/530 emission filters/product/Revvity
Average 90 stars, based on 1 article reviews
530 emission filters - by Bioz Stars, 2026-06
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filters  (Revvity)
90
Revvity filters
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Filters, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/filters/product/Revvity
Average 90 stars, based on 1 article reviews
filters - by Bioz Stars, 2026-06
90/100 stars
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emissions  (Revvity)
90
Revvity emissions
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Emissions, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/emissions/product/Revvity
Average 90 stars, based on 1 article reviews
emissions - by Bioz Stars, 2026-06
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biocontinuum hd imaging filter  (Gatan Inc)
99
Gatan Inc biocontinuum hd imaging filter
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Biocontinuum Hd Imaging Filter, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biocontinuum hd imaging filter/product/Gatan Inc
Average 99 stars, based on 1 article reviews
biocontinuum hd imaging filter - by Bioz Stars, 2026-06
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continuum er gif  (Gatan Inc)
98
Gatan Inc continuum er gif
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Continuum Er Gif, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuum er gif/product/Gatan Inc
Average 98 stars, based on 1 article reviews
continuum er gif - by Bioz Stars, 2026-06
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quantum gatan image filter  (Gatan Inc)
95
Gatan Inc quantum gatan image filter
P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in <t>IncuCyte</t> ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Quantum Gatan Image Filter, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantum gatan image filter/product/Gatan Inc
Average 95 stars, based on 1 article reviews
quantum gatan image filter - by Bioz Stars, 2026-06
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Image Search Results


P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in IncuCyte ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Journal: bioRxiv

Article Title: MITF maintains genome stability in non-melanocytic cell lineages and suppresses Hippo pathway signaling

doi: 10.1101/2025.06.09.658599

Figure Lengend Snippet: P53 is activated upon MITF knockdown in U2OS cells. a Confocal microscopy images and quantification of P53 protein levels after 48h treatment with siCON, siMITF and siP53, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells. Scale: 20μM (one-way-ANOVA, n=3-7). b MITF and p53 mRNA expression in U2OS cells. Cells were treated with indicated siRNAs for 48h, followed by real time qPCR analysis. (unpaired t-test, n=3). c Cell cycle profile analyses of siCON and siMITF treated U2OS cells. Cells were fixed after 48h siRNA treatment, followed by staining of DNA content with 7- aminoactinomycin D (7AAD) and flow cytometry analysis (unpaired t-test, n=4). d Caspase 3/7 activity relative to cell confluency after four days of siRNA treatment (siCON, siMITF, sip53, siMITF + sip53). Cells were incubated with caspase-3/7 red dye after siRNA treatment and imaged in IncuCyte ® live-cell imager (one-way-ANOVA, n=3). e quantification of P53 protein levels after 48h siCON and siMITF treatment and 24h treatment with ATMi, ATRi, AuroraAi or DMSO, followed by immunostaining with antibody targeting P53 and DAPI to visualize nuclear cells (one-way ANOVA, n=3). Data presented as mean ± SD. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: To analyze apoptosis, we used Incucyte ® S3 live-cell imager (Sartorius) and caspase-3/7 red dye (Sartorius, 4704).

Techniques: Knockdown, Confocal Microscopy, Immunostaining, Expressing, Staining, Flow Cytometry, Activity Assay, Incubation